Proteo-lipobeads for the oriented encapsulation of membrane proteins
by Frank, P.; Siebenhofer, B.; Hanzer, T.; Geiss, A. F.; Schadauer, F.; Reiner-Rozman, C.; Durham, B.; Loew, L. M.; Ludwig, B.; Richter, O. M. H.; Nowak, C.; Naumann, R. L. C.
As a surrogate of live cells, proteo-lipobeads are presented, encapsulating functional membrane proteins in a strict orientation into a lipid bilayer. Assays can be performed just as on live cells, for example using fluorescence measurements. As a proof of concept, we have demonstrated proton transport through cytochrome c oxidase.
- Journal
- Soft Matter
- Volume
- 11
- Issue
- 15
- Year
- 2015
- Start Page
- 2906-2908
- URL
- https://dx.doi.org/10.1039/c4sm02646b
- ISBN/ISSN
- 1744-683X
- DOI
- 10.1039/c4sm02646b