Cleavage resistant photoluminescent proteins and applications thereof

by Beitle, Jr., Robert; Mukherjee, Rudra Palash; Kumar, Thallapuranam Krishnaswamy Suresh; Sakon, Joshua; McNabb, David S.

Photoluminescent proteins resistant to protein cleavage reagents are described herein. In being resistant to cleavage reagents, such photoluminesncent proteins can reduce the number of digestion or cleavage products, thereby simplifying quantification and purification of recombinant protein compositions. In some embodiments, a photoluminescent protein described herein comprises an amino acid sequence having an absence of internal methionine residues.

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