Comparison of \"Polarization Inversion with Spin Exchange at Magic Angle\" and \"Geometric Analysis of Labeled Alanines\" methods for transmembrane helix alignment
by Vostrikov, Vitaly V.; Grant, Christopher V.; Daily, Anna E.; Opella, Stanley J.; Koeppe II, R. Erdman
Using the model alpha-helical peptide acletyl-GGALW(5)LALALALALALALW(19)LAGA-ethanolamide ("GWALP23"), we have compared the polarization inversion with spin exchange at magic angle method and geometric analysis of labeled alanines method for estimating the transmembrane helix orientation. For GWALP23 in bilayers of a short lipid, dilauroylphosphatidylcholine, we find general agreement between the two methods, with a static helix tilt of about 11 degrees-13 degrees with respect to the bilayer normal.
- Journal
- Journal of the American Chemical Society
- Volume
- 130
- Issue
- 38
- Year
- 2008
- Start Page
- 12584
- ISBN/ISSN
- 1520-5126; 0002-7863
- PMID
- 18763771
- DOI
- 10.1021/ja803734k