Calibration of microdialysis probes during long term implantation

by Mou, Xiaodun; Lennartz, Michelle R.; Loegering, Daniel J.; Stenken, Julie Ann

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The anal. performance of in vivo sensor implants is a crucial parameter to understand. Microdialysis sampling probes implanted in vivo into male Sprague-Dawley rats were used to det. how the foreign body response affects probe calibration. Dialysis probes with either a polycarbonate (PC) or polyethersulfone (PES) membrane were implanted. Vitamin B12 and antipyrine were internal stds. to test the membrane and capillary permeability towards different sized mols., and 2-deoxyglucose was employed to trace the glucose metab. variation during the elicited foreign body response. Histol. staining was applied to surrounding tissue seven (PC) and twelve days (PES) after implantation. The capsule surrounding the probe blocked the diffusion of relatively large mol. (Vitamin B12) and caused the decrease in glucose collection by cutting of the blood supply. However, it did not affect the local delivery of antipyrine or 2-deoxyglucose (Table 1). NIH EB001441 is acknowledged for funding. on SciFinder(R)]