Domain Organization in the 54-kDa Subunit of the Chloroplast Signal Recognition Particle

by Henderson, Rory C.; Gao, Feng; Jayanthi, Srinivas; Kight, Alicia; Sharma, Priyanka; Goforth, Robyn L.; Heyes, Colin D.; Henry, Ralph L.; Kumar, Thallapuranam Krishnaswamy Suresh

Chloroplast signal recognition particle (cpSRP) is a heterodimer composed of an evolutionarily conserved 54-kDa GTPase (cpSRP54) and a unique 43-kDa subunit (cpSRP43) responsible for delivering light-harvesting chlorophyll binding protein to the thylakoid membrane. While a nearly complete three-dimensional structure of cpSRP43 has been determined, no high-resolution structure is yet available for cpSRP54. In this study, we developed and examined an in silico threedimensional model of the structure of cpSRP54 by homology modeling using cytosolic homologs. Model selection was guided by single-molecule Forster resonance energy transfer experiments, which revealed the presence of at least two distinct conformations. Small angle x-ray scattering showed that the linking region among the GTPase (G-domain) and methionine-rich (M-domain) domains, an M-domain loop, and the cpSRP43 binding C-terminal extension of cpSRP54 are predominantly disordered. Interestingly, the linker and loop segments were observed to play an important role in organizing the domain arrangement of cpSRP54. Further, deletion of the finger loop abolished loading of the cpSRP cargo, light-harvesting chlorophyll binding protein. These data highlight important structural dynamics relevant to cpSRP54's role in the post- and cotranslational signaling processes.

Journal
Biophysical Journal
Volume
111
Issue
6
Year
2016
Start Page
1151-1162
URL
https://dx.doi.org/10.1016/j.bpj.2016.08.004
ISBN/ISSN
1542-0086; 0006-3495
DOI
10.1016/j.bpj.2016.08.004