Expression of a collagen-binding domain fusion protein: Effect of amino acid supplementation, inducer type, and culture conditions

by Fruchtl, McKinzie S.; Sakon, Josh; Beitle, Jr, Robert R.

Collagen binding domain fusion proteins are of significant importance because of their potential as therapeutic biomaterials. In this paper, we investigate the prodn. of such therapeutic proteins via fermn. of Escherichia coli on both an undefined medium and a defined medium. Defined media with amino acid supplementation provided higher amts. of therapeutic protein than undefined media with no supplementation. Addnl., utilizing lactose instead of isopropyl-β-D-thio-galactoside (IPTG) for induction and extending batch time yielded higher amts. of the model therapeutic. © 2015 American Institute of Chem. Engineers Biotechnol. Prog., 2015.

Journal
Biotechnology Progress
Volume
31
Issue
2
Year
2015
Start Page
503
ISBN/ISSN
1520-6033; 8756-7938
DOI
10.1002/btpr.2048