Fabrication of a nanoconjugate for synergistic antibiotic and photothermal treatment of resistant bacteria

by Jenkins, Samir V.; Meeker, Daniel G.; Beenken, Karen E.; Smeltzer, Mark S.; Chen, Jingyi

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Multi-drug resistant bacterial infections present a vexing challenge in modern medicine, with S. aureus being the most prevalent example. The development of new antibiotics eventually leads to the development of new resistant strains. S. aureus's resistance is further augmented by the formation of biofilms at the infection site, which serve to shield some of the bacteria from circulating drugs, allowing them to further proliferate. To combat resistant strains, we have developed a gold nanocage-based system for the simultaneous delivery of antibiotics and photothermal treatment to resistant bacterial infections. Antibiotic is loaded into polymer coated nanocages and the polymer is then conjugated with antibodies for active targeting of the bacteria. Near-IR irradn. is used to induce the photothermal effect, which caused antibiotic release and photothermal killing of cells. The synergistic effect is assessed in vitro using S. aureus cells. The loading mechanism, loading capacity, and photothermal release mechanism will be discussed.