Identification and characterization of thymosin beta 4 in chicken macrophages using whole cell MALDI-TOF
by Kannan, Lakshmi; Rath, Narayan C.; Liyanage, Rohana; Lay Jr, Jackson Oliver
The aim of the study was to det. chicken monocyte- and granulocyte-assocd. peptides and proteins using "whole cell" matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) and to characterize the peptides based on their abundance. The mass spectra showed a prominent peak at m/z 4963 in monocytes/macrophages but not in the granulocytes. Subsequent purifn. and characterization of the m/z 4963 peptide from an avian macrophage cell line HTC, revealed it to be thymosin β-4 (Tβ-4), an actin-modulating peptide. HTC cells when treated with bacterial lipopolysaccharide and peptidoglycan to det. the modulation of Tβ-4 gene expression or its secretion, showed no changes.
- Journal
- Annals of the New York Academy of Sciences
- Volume
- 1112
- Year
- 2007
- Start Page
- 425
- ISBN/ISSN
- 1749-6632; 0077-8923
- DOI
- 10.1196/annals.1415.028