Identification and characterization of thymosin beta 4 in chicken macrophages using whole cell MALDI-TOF
by Kannan, Lakshmi; Rath, Narayan C.; Liyanage, Rohana; Lay Jr, Jackson Oliver
The aim of the study was to det. chicken monocyte- and granulocyte-assocd. peptides and proteins using "whole cell" matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) and to characterize the peptides based on their abundance. The mass spectra showed a prominent peak at m/z 4963 in monocytes/macrophages but not in the granulocytes. Subsequent purifn. and characterization of the m/z 4963 peptide from an avian macrophage cell line HTC, revealed it to be thymosin Î²-4 (TÎ²-4), an actin-modulating peptide. HTC cells when treated with bacterial lipopolysaccharide and peptidoglycan to det. the modulation of TÎ²-4 gene expression or its secretion, showed no changes.
- Annals of the New York Academy of Sciences
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- 1749-6632; 0077-8923