Microchannel immunoassays with electrochemical detection in devices constructed from low temperature co-fired ceramic and screen printed gold

by Fakunle, Eyitayo S. O.; Fritsch, Ingrid

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The authors report a self-contained electrochem. enzyme linked immunosorbent assay in microchannel devices constructed from low temp. co-fired ceramic with screen-printed gold for fast and sensitive assays. No one has previously reported a device constructed from these materials for combined immobilization and electrochem. detection. These low temp. co-fired ceramic microchannel devices used for electrochem. immunoassay contain 4 integrated, individually-addressable screen-printed gold electrodes. The total channel vol. is under 0.8 μl. The entire immunoassembly and electrochem. detection is carried out within the microchannel device-using mouse IgG as the model analyte and electrochem. detection of p-aminophenol is possible within 15 s of introducing the enzyme substrate, p-aminophenyl phosphate into the microchannel ELISA.

Conference
NSTI Nanotech 2005, NSTI Nanotechnology Conference and Trade Show, Anaheim, CA, United States, May 8-12, 2005
Year
2005
Division
466
URL
http://www.nsti.org/publications/Nanotech/2005/pdf/1169.pdf