Comparison of heparin-immobilized vs. antibody-immobilized microspheres for the capture and detection of cytokines during microdialysis sampling

by Duo, Jia; Espinal Cabrera, Randy Francisco; Stenken, Julie Ann

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Heparin-immobilized beads were compared against antibody-immobilized beads for improving the collection of targeted cytokines through microdialysis sampling probes. Heparin offers an advantage over antibodies with respect to its stability and cost. The cytokines, MCP-1 and RANTES, exhibited increased flux through the probes when either heparin-immobilized or antibody-immobilized beads were included in the perfusion fluid. This suggests that heparin-immobilized beads may be a viable alternative to antibody-immobilized beads to increase the flux of select cytokines during microdialysis sampling. 2009 IEEE.

Conference
IEEE/NIH Life Science Systems and Applications Workshop, LiSSA 2009
Year
2009
Division
112
URL
http://ieeexplore.ieee.org/document/4906722/
DOI
10.1109/LISSA.2009.4906722