Evaluation of the Antioxidant and Anticancer Effects of Biodegradable/Biocompatible Chitosan-Alginate Nanoparticles Loaded with Vitamin C
by Al-Ogaidi, I.
A safe, rapid, and easy-to-scale-up method of loading vitamin C (vit C) into chitosan-alginate nanoparticles (CH-Alg NPs) was established. The CH-Alg/vit C NPs were subjected to bath sonication to achieve their nanosize. Transmission electron microscopy of CH-Alg/vit C NPs stained with 1% neutral phosphotungstic acid showed NPs with sizes between 25-30 nm with spherical shapes. High negative zeta potential values (between -34 and -43) were obtained with a zeta analyzer. The main ingredients of the NPs were found to be chitosan and alginate. Various vit C concentrations were incorporated into the NPs to enhance the anticancer property of vit C. The effect of various pH values on the CH-Alg/vit C NPs was also studied to evaluate their degradation in the stomach and intestines. The results showed that pH approximate to 6 was the optimum level for polyionic interaction. The activity of produced NPs was tested against a mouse muscle cancer cell line model in a dose-dependent manner. CH-Alg/vit C was found to present potential anti-cancer activity, with a half-maximal inhibitory concentration (IC50) of 1.1 g /mL for vit C loaded into CH-Alg NPs.
- Journal
- International Journal of Pharmaceutical Research and Allied Sciences
- Volume
- 7
- Issue
- 3
- Year
- 2018
- Start Page
- 189-197
- URL
- https://www.webofscience.com/wos/woscc/full-record/WOS:000453374400021
- ISBN/ISSN
- 2277-3657