trans-Platinum(II) Thionate Complexes: Synthesis, Structural Characterization, and in vitro Biological Assessment as Potent Anticancer Agents
by Sakamaki, Y.; Mirsadeghi, H. A.; Fereidoonnezhad, M.; Mirzaei, F.; Dehkordi, Z. M.; Chamyani, S.; Alshami, M.; Abedanzadeh, S.; Shahsavari, H. R.; Beyzavi, M. H.
A series of Pt(II) complexes trans-[Pt(PPh(2)allyl)(2)(kappa(1)-S-SR)(2)], 1, PPh(2)allyl=allyldiphenylphosphine, SR=pyridine-2-thiol (Spy, 1 a), 5-(trifluoromethyl)-pyridine-2-thiol (SpyCF(3)-5, 1 b), pyrimidine-2-thiol (SpyN, 1 c), benzothiazole-2-thiol (Sbt, 1 d), benzimidazole-2-thiol (Sbi, 1 e), were synthesized. They were characterized by NMR, HR ESI-MS, and X-ray crystallography. Treatment of human cancer cell lines (A549, SKOV3, MCF-7) with these complexes resulted in promising antitumor effects in comparison with cisplatin. These compounds showed suitable selectivity between tumorigenic and non-tumorigenic (MCF-10 A) cell lines. Analyses of cell cycle progression and apoptosis were conducted for 1 a, the most cytotoxic compound, to screen dose/time response and to study the antiproliferative mechanism. An electrophoresis mobility shift assay was performed to assess the direct interaction of 1 a with DNA and the strong genotoxic ability was indicated through the comet assay method.
- Journal
- ChemPlusChem
- Volume
- 84
- Issue
- 10
- Year
- 2019
- Start Page
- 1525-1535
- URL
- https://dx.doi.org/10.1002/cplu.201900394
- ISBN/ISSN
- 2192-6506
- DOI
- 10.1002/cplu.201900394