Design of a ruthenium-labeled cytochrome c derivative to study electron transfer with the cytochrome bc(1) complex
by Engstrom, Gregory; Rajagukguk, Ray; Saunders, Aleister J.; Patel, Chetan N.; Rajagukguk, Sany; Merbitz-Zahradnik, Torsten; Xiao, Kunhong; Pielak, Gary J.; Trumpower, Bernard; Yu, Chang-An; Yu, Linda; Durham, Bill; Millett, Francis
A new ruthenium-cytochrome c derivative was designed to study electron transfer from cytochrome bc(1) to cytochrome c (Cc). The single sulfhydryl on yeast H39C;C102T iso-1-Cc was labeled with Ru(2,2'-bipyrazine)(2)(4-bromomethyl-4'-methyl-2,2'-bipyridine) to form Ru-z-39-Cc. The Ru-z-39-Cc derivative has the same steady-state activity with yeast cytochrome bc(1) as wild-type yeast iso-1-Cc, indicating that the ruthenium complex does not interfere in the binding interaction. Laser excitation of reduced Ru-z-39-Cc results in electron transfer from heme c to the excited state of ruthenium with a rate constant of 1.5 x 10(6) s(-1). The resulting Ru(I) is rapidly oxidized by atmospheric oxygen in the buffer. The yield of photooxidized heme c is 20% in a single flash. Flash photolysis of a 1:1 complex between reduced yeast cytochrome bc(1) and Ru-z-39-Cc at low ionic strength leads to rapid photooxidation of heme c, followed by intracomplex electron transfer from cytochrome cl to heme c with a rate constant of 1.4 x 10(4) s(-1). As the ionic strength is raised above 100 mM, the intracomplex phase disappears, and a new phase appears due to the bimolecular reaction between solution Ru-39-Cc and cytochrome bc(1). The interaction of yeast Ru-39-Cc with yeast cytochrome bc(1) is stronger than that of horse Ru-39-Cc with bovine cytochrome bc(1), suggesting that nonpolar interactions are stronger in the yeast system.
- Journal
- Biochemistry
- Volume
- 42
- Issue
- 10
- Year
- 2003
- Start Page
- 2816-2824
- URL
- https://dx.doi.org/10.1021/bi027213g
- ISBN/ISSN
- 1520-4995; 0006-2960
- DOI
- 10.1021/bi027213g