Brominated Graphene as Mimetic Peroxidase for Sulfide Ion Recognition

by Singh, S.; Mitra, K.; Shukla, A.; Singh, R.; Gundampati, R. K.; Misra, N.; Maiti, P.; Ray, B.

Brominated graphene (GBR) with-,3% bromine content has shown novel peroxidase mimetic activity toward.3,3',5,5'-tetramethylbenzidine (TMB) in the presence of H2O2. Optimum activity has been observed at pH 4.48 and after a minimum similar to 30 min of equilibration time. Among the different analVtes studied using the sensor combining TMB,. H2O2, and GBR in phosphate buffer of pH 4.48, the S2- ion has effectively shown a short duration of sensing (similar to 2 min) within the detection range of 0.04-1 m.M. A calibration curve for S2- ion estimation has been constructed with the experimental linearity in 0.04-0.4 mM range and having the limit of detection (LOD) value of 25.3 mu M. A standard addition experiment has validated the method. A paper strip sensor has been fabricated for successful detection of S2- ion.

Journal
Analytical Chemistry
Volume
89
Issue
1
Year
2017
Start Page
783-791
URL
https://dx.doi.org/10.1021/acs.analchem.6b03535
ISBN/ISSN
1520-6882; 0003-2700
DOI
10.1021/acs.analchem.6b03535