Molecule-specific darkfield and multiphoton imaging using gold nanocages
by Powless, A. J.; Jenkins, S. V.; McKay, M. L.; Jingyi, Chen; Muldoon, T. J.
Due to their robust optical properties, biological inertness, and readily adjustable surface chemistry, gold nanostructures have been demonstrated as contrast agents in a variety of biomedical imaging applications. One application is dynamic imaging of live cells using bioconjugated gold nanoparticles to monitor molecule trafficking mechanisms within cells; for instance, the regulatory pathway of epidermal growth factor receptor (EGFR) undergoing endocytosis. In this paper, we have demonstrated a method to track endocytosis of EGFR in MDA-MB-468 breast adenocarcinoma cells using bioconjugated gold nanocages (AuNCs) and multiphoton microscopy. Dynamic imaging was performed using a time series capture of 4 images every minute for one hour. Specific binding and internalization of the bioconjugated AuNCs was observed while the two control groups showed non-specific binding at fewer surface sites, leading to fewer bound AuNCs and no internalization.
- Conference
- Reporters, Markers, Dyes, Nanoparticles and Molecular Probes for Biomedical Applications VII
- Year
- 2015
- URL
- https://dx.doi.org/10.1117/12.2080152
- ISBN/ISSN
- 1996-756X; 0277-786X; 9781628414295
- DOI
- 10.1117/12.2080152