Matrix-assisted ionization Fourier transform mass spectrometry for the analysis of lipids
by Liyanage, R.; Gidden, J.; Wilkins, C. L.; Lay, J. O.
Rationale Assessing the utility of vacuum matrix-assisted ionization (MAI) for the direct and rapid analysis of lipids in complex samples with emphasis on bacterial taxonomy. Methods Matrix-assisted ionization Fourier transform mass spectrometry (MAI-FTMS) was used to characterize polar and non-polar lipids in mixtures. Results For non-polar lipid triacylglycerols (TAGs), MAI-FTMS produced lipid-specific ions for eight different edible oils and allowed these oils to be identified based on their MAI-FTMS profiles. For polar lipids from bacteria, MAI-FTMS of crude lipid extracts allowed taxonomic identification of eight blind-coded samples based on taxonomy-specific phospholipid profiles. MAI produced results comparable and complementary to benchmark MALDI and ESI methods currently used for characterization of polar and non-polar lipids in the same mixtures. Conclusions The newly developed MAI technique is a rapid, simple and complementary method for the characterization of polar and non-polar lipids in complex mixtures.
- Journal
- Rapid Communications in Mass Spectrometry
- Volume
- 35
- Issue
- S1
- Year
- 2021
- URL
- https://dx.doi.org/10.1002/rcm.8349
- ISBN/ISSN
- 1097-0231; 0951-4198
- DOI
- 10.1002/rcm.8349