Comparison of heparin-immobilized vs. antibody-immobilized microspheres for the capture and detection of cytokines during microdialysis sampling
by Duo, J.; Espinal Cabrera, R. F.; Stenken, J. A.
Heparin-immobilized beads were compared against antibody-immobilized beads for improving the collection of targeted cytokines through microdialysis sampling probes. Heparin offers an advantage over antibodies with respect to its stability and cost. The cytokines, MCP-1 and RANTES, exhibited increased flux through the probes when either heparin-immobilized or antibody-immobilized beads were included in the perfusion fluid. This suggests that heparin-immobilized beads may be a viable alternative to antibody-immobilized beads to increase the flux of select cytokines during microdialysis sampling.
- Conference
- IEEE/NLM Life Science Systems and Applications Workshop (LiSSA 2009)
- Year
- 2009
- Division
- 112-115
- URL
- https://dx.doi.org/10.1109/lissa.2009.4906722
- ISBN/ISSN
- 9781424442928
- DOI
- 10.1109/lissa.2009.4906722