Distribution coefficient of rifabutin in liposome/water system as measured by different methods
by Vostrikov, V. V.; Selishcheva, A. A.; Sorokoumova, G. M.; Shakina, Y. N.; Shvets, V. I.; Savel'ev, O. Y.; Polshakov, V. I.
Distribution coefficient (D) of rifabutin in liposome/water system was measured by phase separation and fluorescence probe quenching techniques. D values. were identical suggesting that rifabutin is fully immersed into lipid bilayer. Structural studies of phospholipid bilayer employing P-31 NMR spectroscopy demonstrated that introduction of rifabutin does not alter the bilayer structure. A scheme of the rifabutin position in lipid bilayer based on the calculated size of rifabutin molecule is proposed.
- Journal
- European Journal of Pharmaceutics and Biopharmaceutics
- Volume
- 68
- Issue
- 2
- Year
- 2008
- Start Page
- 400-405
- URL
- https://dx.doi.org/10.1016/j.ejpb.2007.05.014
- ISBN/ISSN
- 1873-3441; 0939-6411
- DOI
- 10.1016/j.ejpb.2007.05.014