Imaging Dynamic Processes in Multiple Dimensions and Length Scales

by Filbrun, S. L.; Zhao, F.; Chen, K. C.; Huang, T. X.; Yang, M.; Cheng, X. D.; Dong, B.; Fang, N.

Optical microscopy has become an invaluable tool for investigating complex samples. Over the years, many advances to optical microscopes have been made that have allowed us to uncover new insights into the samples studied. Dynamic changes in biological and chemical systems are of utmost importance to study. To probe these samples, multidimensional approaches have been developed to acquire a fuller understanding of the system of interest. These dimensions include the spatial information, such as the three-dimensional coordinates and orientation of the optical probes, and additional chemical and physical properties through combining microscopy with various spectroscopic techniques. In this review, we survey the field of multidimensional microscopy and provide an outlook on the field and challenges that may arise.

Journal
Annual Review of Physical Chemistry
Volume
73
Year
2022
Start Page
377-402
URL
https://dx.doi.org/10.1146/annurev-physchem-090519-034100
ISBN/ISSN
1545-1593; 0066-426X
DOI
10.1146/annurev-physchem-090519-034100