Matrix-assisted ionization Fourier transform mass spectrometry for the analysis of lipids

by Liyanage, Rohana; Gidden, Jennifer; Wilkins, Charles L.; Lay Jr, Jackson Oliver

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Rationale Assessing the utility of vacuum matrix-assisted ionization (MAI) for the direct and rapid analysis of lipids in complex samples with emphasis on bacterial taxonomy. Methods Matrix-assisted ionization Fourier transform mass spectrometry (MAI-FTMS) was used to characterize polar and non-polar lipids in mixtures. Results For non-polar lipid triacylglycerols (TAGs), MAI-FTMS produced lipid-specific ions for eight different edible oils and allowed these oils to be identified based on their MAI-FTMS profiles. For polar lipids from bacteria, MAI-FTMS of crude lipid extracts allowed taxonomic identification of eight blind-coded samples based on taxonomy-specific phospholipid profiles. MAI produced results comparable and complementary to benchmark MALDI and ESI methods currently used for characterization of polar and non-polar lipids in the same mixtures. Conclusions The newly developed MAI technique is a rapid, simple and complementary method for the characterization of polar and non-polar lipids in complex mixtures.

Journal
Rapid Communications in Mass Spectrometry
Volume
35
Year
2021
Start Page
e8349
ISBN/ISSN
0951-4198; 1097-0231
PMID
30421829
DOI
10.1002/rcm.8349