Chemiluminescent detection of amino acids

by Bobbitt, Donald R.; Brune, Stephen N.

A detection system for determining the quantity of amino acid in a sample stream is provided based on the reaction of a buffer at pH 10-11 with a reagent, Ru(bpy)33+, which is generated electrochem. on site. The detection system is further characterized by immediate luminescence upon reaction of the buffer in the pH range containing amino acid, with the reagent Ru(bpy)33+. The detection system is capable of not only immediate detection of the quantity of amino acid in a sample stream, but even in very low concentrations A schematic representation of the detection system is shown. A table lists the relative luminescence efficiency for 8 amino acids tested with the system.

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