Alternate carbohydrate and nontraditional inducer leads to increased productivity of a collagen binding domain fusion protein via fed-batch fermentation

by Fruchtl, McKinzie S.; Sakon, Josh; Beitle, Jr, Robert R.

The production of collagen binding domain fusion proteins is of significant importance because of their potential as therapeutic biomaterials. It was previously reported that the expression of collagen-binding domain fusion proteins in Escherichia coil was higher when expressed using lactose as an inducer and chemically defined growth media on a shake flask scale. In an effort to further investigate factors that affect expression levels on a fed-batch scale, alternative induction techniques were tested in conjunction with fed-batch fermentation. In this paper, we discuss ten fed-batch fermentation experiments utilizing either glucose or glycerol feed and using lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG) as an induction source. It was found that glycerol-fed fermentations induced with lactose allowed for greater expression of target protein, though lesser cell densities were achieved. (C) 2016 Elsevier B.V. All rights reserved.

Journal
Journal of Biotechnology
Volume
226
Year
2016
Start Page
65
ISBN/ISSN
1873-4863; 0168-1656
DOI
10.1016/j.jbiotec.2016.03.016