Proteo-lipobeads for the oriented encapsulation of membrane proteins

by Frank, Pinar; Siebenhofer, Bernhard; Hanzer, Theresa; Geiss, Andreas F.; Schadauer, Florian; Reiner-Rozman, Ciril; Durham, Bill; Loew, Leslie M.; Ludwig, Bernd; Richter, Oliver-M. H.; Nowak, Christoph; Naumann, Renate L. C.

As a surrogate of live cells, proteo-lipobeads are presented, encapsulating functional membrane proteins in a strict orientation into a lipid bilayer. Assays can be performed just as on live cells, for example using fluorescence measurements. As a proof of concept, we have demonstrated proton transport through cytochrome c oxidase.

Journal
Soft Matter
Volume
11
Issue
15
Year
2015
Start Page
2906
ISBN/ISSN
1744-683X
PMID
25763882
DOI
10.1039/c4sm02646b