Proteo-lipobeads for the oriented encapsulation of membrane proteins
by Frank, Pinar; Siebenhofer, Bernhard; Hanzer, Theresa; Geiss, Andreas F.; Schadauer, Florian; Reiner-Rozman, Ciril; Durham, Bill; Loew, Leslie M.; Ludwig, Bernd; Richter, Oliver-M. H.; Nowak, Christoph; Naumann, Renate L. C.
As a surrogate of live cells, proteo-lipobeads are presented, encapsulating functional membrane proteins in a strict orientation into a lipid bilayer. Assays can be performed just as on live cells, for example using fluorescence measurements. As a proof of concept, we have demonstrated proton transport through cytochrome c oxidase.
- Journal
- Soft Matter
- Volume
- 11
- Issue
- 15
- Year
- 2015
- Start Page
- 2906
- ISBN/ISSN
- 1744-683X
- PMID
- 25763882
- DOI
- 10.1039/c4sm02646b