NMR Methods to Characterize Protein-Ligand Interactions

by Maity, S.; Gundampati, R. K.; Kumar, T. K. S.

Structural information pertaining to the interactions between biological macromolecules and ligands is of potential significance for understanding of molecular mechanisms in key biological processes. Recently, nuclear magnetic resonance (NMR) spectroscopic techniques has come of age and has widened its scope to characterize binding interactions of small molecules with biological macromolecules especially, proteins. NMR spectroscopy-based techniques are versatile due to their ability to examine weak binding interactions and for rapid screening the binding affinities of ligands with proteins at atomic resolution. In this review, we provide a broad overview of some of the important NMR approaches to investigate interactions of small organic molecules with proteins.

Journal
Natural Product Communications
Volume
14
Issue
5
Year
2019
Start Page
17
URL
https://dx.doi.org/10.1177/1934578x19849296
ISBN/ISSN
1555-9475; 1934-578X
DOI
10.1177/1934578x19849296