Bioanalytical chemistry of cytokines - A review

by Stenken, Julie Ann; Poschenrieder, Andreas J.

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Cytokines are bioactive proteins produced by many different cells of the immune system. Due to their role in different inflammatory disease states and maintaining homeostasis, there is enormous clinical interest in the quantitation of cytokines. The typical standard methods for quantitation of cytokines are immunoassay-based techniques including enzyme-linked immusorbent assays (ELISA) and bead-based immunoassays read by either standard or modified flow cytometers. A review of recent developments in analytical methods for measurements of cytokine proteins is provided. This review briefly covers cytokine biology and the analysis challenges associated with measurement of these biomarker proteins for understanding both health and disease. New techniques applied to immunoassay-based assays are presented along with the uses of aptamers, electrochemistry, mass spectrometry, optical resonator-based methods. Methods used for elucidating the release of cytokines from single cells as well as in vivo collection methods are described. (C) 2014 Elsevier B.V. All rights reserved.

Journal
Analytica Chimica Acta
Volume
853
Year
2015
Start Page
95
ISBN/ISSN
0003-2670
PMID
25467452
DOI
10.1016/j.aca.2014.10.009