Comparison of microdialysis sampling perfusion fluid components on the foreign body reaction in rat subcutaneous tissue

by Keeler, Geoffrey David; Durdik, Jeannine M.; Stenken, Julie Ann

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Microdialysis sampling is a commonly used technique for collecting solutes from the extracellular space of tissues in lab. animals and humans. Large mol. wt. solutes can be collected using high mol. wt. cutoff (MWCO) membranes (100 kDa or greater). High MWCO membranes require addn. of high mol. wt. dextrans or albumin to the perfusion fluid to prevent fluid loss via ultrafiltration. While these perfusion fluid additives are commonly used during microdialysis sampling, the tissue response to the loss of these compds. across the membrane is poorly understood. Tissue reactions to implanted microdialysis sampling probes contg. different microdialysis perfusion fluids were compared over a 7-day time period in rats. The base perfusion fluid was Ringer's soln. supplemented with either bovine serum albumin (BSA), rat serum albumin (RSA), Dextran-70, or Dextran-500. A significant inflammatory response to Dextran-70 was obsd. No differences in the tissue response between BSA and RSA were obsd. Among these agents, the BSA, RSA, and Dextran-500 produced a significantly reduced inflammatory response compared to the Dextran-70. This work demonstrates that use of Dextran-70 in microdialysis sampling perfusion fluids should be eliminated and replaced with Dextran-500 or other alternatives.

Journal
European Journal of Pharmaceutical Sciences
Volume
57
Year
2014
Start Page
60
ISBN/ISSN
0928-0987
PMID
24239995
DOI
10.1016/j.ejps.2013.11.005