Trapping of wide range mass-to-charge ions and dependence on matrix amount in internal source MALDI-FTMS

by Jaber, Arwah J.; Kaufman, Jacob M.; Liyanage, Rohana; Akhmetova, Evgenia; Marney, Samuel; Wilkins, Charles L.

Internal ionization source MALDI-FTMS shows clear variation of number average molecular weight (M-n) for an equimolar mixture of four PEG polymers (PEG 2000, PEG 4000, PEG 6000, PEG 8000) when the 2,5-dihydroxy benzoic acid (DHB) matrix to PEG ratio is varied or the laser power is changed. As the matrix to analyte ratio is increased, the analyte signal of higher molecular mass ions increases and the signal of lower mass ions decreases. Laser power dependence studies show a similar trend. Possible explanations for these observations are discussed. (c) 2005 American Society for Mass Spectrometry.

Journal
Journal of the American Society for Mass Spectrometry
Volume
16
Issue
11
Year
2005
Start Page
1772
ISBN/ISSN
1879-1123; 1044-0305
DOI
10.1016/j.jasms.2005.06.014