Resolving the Heterogeneous Adsorption of Antibody Fragment on a 2D Layered Molybdenum Disulfide by Super-Resolution Imaging

by Huang, T. X.; Yang, M.; Giang, H.; Dong, B.; Fang, N.

The development of nanomaterials such as twodimensional (2D) layered materials advanced applications in many fields, including biosensors format based on field-effect transistors. The unique physical and chemical properties of 2D layered materials enable the detection limit of biomolecules as low as similar to 1 pg/mL. The majority of 2D layered materials contain different structural features and defects introduced in chemical synthesis and fabrication processing. These structural features have different physicochemical properties, causing heterogeneous adsorption of bioreceptors like antibodies, enzymes, etc. Understanding the correlation between the adsorption of bioreceptors and properties of structural features is essential for building highly efficient, sensitive biosensors based on 2D layered materials. Here, we utilize a single-molecule localization-based super-resolved fluorescence imaging method to unveil the inhomogeneous adsorption of antibody fragments on 2D layered molybdenum disulfide (MoS2). The surface coverage of antibody fragments on MoS2 thin flakes is quantitatively measured and compared at different structural features and different layer thicknesses. The methodology in the current work can be extended to study bioreceptor adsorption on other types of 2D layered materials and pave a way to improve biosensors' sensitivity based on defect engineering 2D layered materials.

Journal
Langmuir
Volume
38
Issue
24
Year
2022
Start Page
7455-7461
URL
https://dx.doi.org/10.1021/acs.langmuir.2c00420
ISBN/ISSN
1520-5827; 0743-7463
DOI
10.1021/acs.langmuir.2c00420