TiO2 nanowire bundle microelectrode based impedance immunosensor for rapid and sensitive detection of Listeria monocytogenes
by Wang, Ronghui; Dong, Wenjun; Ruan, Chuanmin; Kanayeva, Damira Ashimkhanovna; Lassiter, Kentu; Tian, Z. Ryan; Li, Yanbin
A novel TiO2 nanowire bundle microelectrode based immunosensor was demonstrated as a more sensitive, specific, and rapid technology for detection of Listeria monocytogenes. TiO2 nanowire bundle was prepared through a hydrothermal reaction of alkali with TiO2 powder and connected to gold microelectrodes with mask welding. Monoclonal antibodies were immobilized on the surface of a TiO2 nanowire bundle to specifically capture L. monocytogenes. Impedance change caused by the nanowire-antibody-bacteria complex was measured and correlated to bacterial number. This nanowire bundle based immunosensor could detect as low as 10(2) cfu/ml of L. monocytogenes in 1 h without significant interference from other foodborne pathogens.
- Nano Letters
- Start Page
- 1530-6992; 1530-6984