Immunoelectrochemical assay in combination with homogeneous enzyme-labeled antibody conjugation for rapid detection of Salmonella
by Yang, Zhongping; Li, Y. B.; Balagtas, Christopher Sagala; Slavik, Michael F.; Paul, David W.
An immunoelectrochemical assay in combination with homogeneous enzyme-labeled antibody conjugation was developed for rapid detection of Salmonella. The assay was performed by mixing alkaline phosphatase linked anti-Salmonella (APLAS) with Salmonella in a solution. The Salmonella-APLAS conjugate separated by polycarbonate membrane filtration was incubated with a phenyl phosphate substrate to produce phenol. The concentration of Salmonella cells was determined by measuring phenol oxidation peak current using differential pulse voltammetry at a renewable carbon paste electrode. This assay could be completed within two hours with a detection limit of 5 x 10(3) cells/mL. A linear response was found for Salmonella between 5 x 10(3) and 1 x 10(6) cells/mL.
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