Immunoelectrochemical assay in combination with homogeneous enzyme-labeled antibody conjugation for rapid detection of Salmonella

by Yang, Zhongping; Li, Y. B.; Balagtas, Christopher Sagala; Slavik, Michael F.; Paul, David W.

An immunoelectrochemical assay in combination with homogeneous enzyme-labeled antibody conjugation was developed for rapid detection of Salmonella. The assay was performed by mixing alkaline phosphatase linked anti-Salmonella (APLAS) with Salmonella in a solution. The Salmonella-APLAS conjugate separated by polycarbonate membrane filtration was incubated with a phenyl phosphate substrate to produce phenol. The concentration of Salmonella cells was determined by measuring phenol oxidation peak current using differential pulse voltammetry at a renewable carbon paste electrode. This assay could be completed within two hours with a detection limit of 5 x 10(3) cells/mL. A linear response was found for Salmonella between 5 x 10(3) and 1 x 10(6) cells/mL.

Journal
Electroanalysis
Volume
10
Issue
13
Year
1998
Start Page
913
ISBN/ISSN
1521-4109; 1040-0397
DOI
10.1002/(SICI)1521-4109(199810)10:13<913::AID-ELAN913>3.3.CO;2-S