Control of reaction chemoselectivity with a circular DNA template in the ligation of short oligodeoxyribonucleotides
by Selvasekaran, J.; Turnbull, K. D.
A study has been conducted to examine the chemoselective control attained in the ligation of short oligodeoxyribonucleotides (ODNs) directed by association on a circular DNA template through triplex formation relative to the same ligation on a single-strand DNA template through duplex formation. The highly efficient, chemospecific ligation achieved under a variety of conditions on the circular DNA template presumably arises from the higher substrate-template association resulting in improved transition state accessibility and protection of the ODNs from side reactions.
- Journal
- Bioorganic and Medicinal Chemistry
- Volume
- 9
- Issue
- 9
- Year
- 2001
- Start Page
- 2493-2500
- URL
- https://dx.doi.org/10.1016/s0968-0896(01)00215-2
- ISBN/ISSN
- 1464-3391; 0968-0896
- DOI
- 10.1016/s0968-0896(01)00215-2