An electrophoretic mobility shift assay for methionine sulfoxide in proteins

by Saunders, Christopher Chapin; Stites, Wesley Eugene

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Study of the posttranslational modification of methionine to its sulfoxide has been receiving increasing attention because of its implication in regulation of protein activity, but techniques for the detection of this modification remain limited. In particular, there has been no method to detect the oxidation of methionine on polyacrylamide gels. Here we demonstrate that alkylation of methionine introduces a charge change that shifts the mobility of the protein on an acidic gel relative to the alkylation-resistant sulfoxide form. (C) 2011 Elsevier Inc. All rights reserved.

Journal
Analytical Biochemistry
Volume
421
Issue
2
Year
2012
Start Page
767
ISBN/ISSN
0003-2697
PMID
22230286
DOI
10.1016/j.ab.2011.12.021