Mass spectrometric study of noncovalent gas phase interaction of staphylococcal nucleases with 5 '-nucleoside phosphate inhibitors

by Gong, Weihong; Polkowski, II, Gregory G.; Fritsch, Ingrid; Wilkins, Charles L.

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A series of electrospray ionization (ESI) mass spectral studies of noncovalent gas phase complexes of staphylococcal nucleases (SNases) and tailored SNase mutants with 5'-nucleoside phosphate inhibitors are reported. Both quadrupole ion trap (IT) and external source Fourier transform mass spectrometers (FTMS) were used to obtain low and high resolution results, respectively. It is concluded that competitive binding comparisons are possible with FTMS, but not quadrupole IT mass analysis, due to the need for the high resolving power provided by the FTMS mass analyzer. It is found that SNase-inhibitor complexes can be observed. In contrast to previous solution studies and X-ray crystallography results, the gas phase complexes do not contain Ca ions.

Journal
International Journal of Mass Spectrometry
Volume
222
Issue
1-3
Year
2003
Start Page
397
ISBN/ISSN
1387-3806
DOI
10.1016/S1387-3806(02)00995-8