Mass spectrometric study of noncovalent gas phase interaction of staphylococcal nucleases with 5 '-nucleoside phosphate inhibitors
by Gong, Weihong; Polkowski, II, Gregory G.; Fritsch, Ingrid; Wilkins, Charles L.
A series of electrospray ionization (ESI) mass spectral studies of noncovalent gas phase complexes of staphylococcal nucleases (SNases) and tailored SNase mutants with 5'-nucleoside phosphate inhibitors are reported. Both quadrupole ion trap (IT) and external source Fourier transform mass spectrometers (FTMS) were used to obtain low and high resolution results, respectively. It is concluded that competitive binding comparisons are possible with FTMS, but not quadrupole IT mass analysis, due to the need for the high resolving power provided by the FTMS mass analyzer. It is found that SNase-inhibitor complexes can be observed. In contrast to previous solution studies and X-ray crystallography results, the gas phase complexes do not contain Ca ions.
- International Journal of Mass Spectrometry
- Start Page
- 1873-2798; 1387-3806