Genome-wide Quantification of the Effect of Gene Overexpression on Escherichia coli Growth
by Chen, Hao; Venkat, Sumana; Wilson, Jessica; McGuire, Paige; Chang, Abigail L.; Gan, Qinglei; Fan, Chenguang
Recombinant protein production plays an essential role in both biological studies and pharmaceutical production. Escherichia coli is one of the most favorable hosts for this purpose. Although a number of strategies for optimizing protein production have been developed, the effect of gene overexpression on host cell growth has been much less studied. Here, we performed high-throughput tests on the E. coli a complete set of E. coli K-12 ORF archive (ASKA) collection to quantify the effects of overexpressing individual E. coli genes on its growth. The results indicated that overexpressing membrane-associated proteins or proteins with high abundances of branched-chain amino acids tended to impair cell growth, the latter of which could be remedied by amino acid supplementation. Through this study, we expect to provide an index for a fast pre-study estimate of host cell growth in order to choose proper rescuing approaches when working with different proteins.
- Journal
- Genes
- Volume
- 9
- Issue
- 8
- Year
- 2018
- URL
- https://dx.doi.org/10.3390/genes9080414
- ISBN/ISSN
- 2073-4425
- DOI
- 10.3390/genes9080414