trans-Platinum(II) Thionate Complexes: Synthesis, Structural Characterization, and in vitro Biological Assessment as Potent Anticancer Agents
by Sakamaki, Yoshie; Mirsadeghi, Hasti Ahmadi; Fereidoonnezhad, Masood; Mirzaei, Faezeh; Dehkordi, Zahra Moghimi; Chamyani, Samira; Alshami, Mia; Abedanzadeh, Sedigheh; Shahsavari, Hamid R.; Beyzavi, M. Hassan
A series of Pt(II) complexes trans-[Pt(PPh(2)allyl)(2)(kappa(1)-S-SR)(2)], 1, PPh(2)allyl=allyldiphenylphosphine, SR=pyridine-2-thiol (Spy, 1 a), 5-(trifluoromethyl)-pyridine-2-thiol (SpyCF(3)-5, 1 b), pyrimidine-2-thiol (SpyN, 1 c), benzothiazole-2-thiol (Sbt, 1 d), benzimidazole-2-thiol (Sbi, 1 e), were synthesized. They were characterized by NMR, HR ESI-MS, and X-ray crystallography. Treatment of human cancer cell lines (A549, SKOV3, MCF-7) with these complexes resulted in promising antitumor effects in comparison with cisplatin. These compounds showed suitable selectivity between tumorigenic and non-tumorigenic (MCF-10 A) cell lines. Analyses of cell cycle progression and apoptosis were conducted for 1 a, the most cytotoxic compound, to screen dose/time response and to study the antiproliferative mechanism. An electrophoresis mobility shift assay was performed to assess the direct interaction of 1 a with DNA and the strong genotoxic ability was indicated through the comet assay method.
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