Identification and quantification of glycoside flavonoids in the energy crop Albizia julibrissin

by Lau, Ching S.; Carrier, Danielle J.; Beitle, Robert R.; Bransby, David I.; Howard, Luke R.; Lay, Jackson O.; Liyanage, Rohana; Clausen, Edgar C.

Oxygen radical absorbance capacity (ORAC) values showed that methanolic extracts of Albizia julibrissin foliage displayed antioxidant activity. High performance liquid chromatography (HPLC) and mass spectrometry (MS) techniques were utilized in the identification of the compounds. The analysis confirmed the presence of three compounds in A. julibrissin foliage methanolic extract: an unknown quercetin derivative with mass of 6 10 Da, hyperoside (quercetin-3-O-galactoside), and quercitrin (quercetin-3-O-rhamnoside). Fast performance liquid chromatography (FPLC) was employed to fractionate the crude A. julibrissin foliage methanolic extract into its individual flavonoid components. The flavonoids were quantified in terms of mass and their respective contribution to the overall ORAC value. Quercetin glycosides accounted for 2.0% of total foliage.

Journal
Bioresource Technology
Volume
98
Issue
2
Year
2007
Start Page
429-435
URL
https://dx.doi.org/10.1016/j.biortech.2005.12.011
ISBN/ISSN
1873-2976; 0960-8524
DOI
10.1016/j.biortech.2005.12.011