Production of an anti-Candida peptide via fed batch and ion exchange chromatography
by Mukherjee, Rudra Palash; Beitle, Robert; Jayanthi, Srinivas; Kumar, T. K. S.; McNabb, David S.
Interest in peptides as diagnostic and therapeutic materials require their manufacture via either a recombinant or synthetic route. This study examined the former, where a recombinant fusion consisting of an antifungal peptide was expressed and isolated from Escherichia coli. Fed batch fermentation with E. coli harboring an arabinose-inducible plasmid produced the 12 residue anti-Candida peptide fused to the N-terminal of Green Fluorescent Protein (GFP(UV)). The purification of the fusion protein, using ion-exchange chromatography, was monitored by using the intrinsic fluorescence of GFP(UV). The recombinant antifungal peptide was successfully released by cyanogen bromide-induced cleavage of the fusion protein. The recombinant peptide showed the expected antifungal activity.