Instrumentation for Automated-Determination of Protein Stability

by Stites, Wesley Eugene; Byrne, Michael Patrick; Aviv, J.; Kaplan, M.; Curtis, P. M.

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A new instrument system has been developed that automatically carries out solvent and thermal denaturations of proteins using fluorescence as a probe of structure. This instrument also automatically performs pH titrations and can make kinetic measurements on the time scale of seconds. The design philosophy and implementation are described. The prototype instrument was subjected to extensive testing. The instrument can very reproducibly and accurately collect data that allow the calculation of the thermodynamics of protein denaturation. This data collection can proceed without human intervention after acquisition start until the data are saved. (C) 1995 Academic Press, Inc.

Journal
Analytical Biochemistry
Volume
227
Issue
1
Year
1995
Start Page
112
ISBN/ISSN
0003-2697
DOI
10.1006/abio.1995.1259