CHBC Faculty Publications

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Author: Stites, Wesley Eugene

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TitlePublication NamePub. TypeYear
Investigation of Methionine Sulfoxide Formation as a Regulator of ProteolysisBiophysical JournalAbstract2017
Methionine Sulfoxide Formation by Cigarette Smoke is Associated with the Degradation of Plasma ProteinsBiophysical JournalAbstract2017
Expression and purification of recombinant Von Willebrand factor A1A2A3 domainsFEBS JournalAbstract2015
The fluorescence detected guanidine hydrochloride equilibrium denaturation of wild-type staphylococcal nuclease does not fit a three-state unfolding modelBiochimieJournal Article2013
The pH dependence of staphylococcal nuclease stability is incompatible with a three-state denaturation modelBiophysical ChemistryJournal Article2013
An electrophoretic mobility shift assay for methionine sulfoxide in proteinsAnalytical BiochemistryJournal Article2012
Theory of the protein equilibrium population snapshot by HID exchange electrospray ionization mass spectrometry (PEPS-HDX-ESI-MS) method used to obtain protein folding energies/rates and selected supporting experimental evidenceInternational Journal of Mass SpectrometryJournal Article2012
Thermodynamic principles for the engineering of pH-driven conformational switches and acid insensitive proteinsBiophysical ChemistryJournal Article2011
Comparison of two ESI-MS based H/D exchange methods for extracting protein folding energiesInternational Journal of Mass SpectrometryJournal Article2009
Excluded volume effects upon protein stability in covalently crosslinked proteins with variable linker lengthsAbstracts of Papers, 235th ACS National Meeting, New Orleans, LA, United States, April 6-10, 2008Abstract2008
Effects of excluded volume upon protein stability in covalently cross-linked proteins with variable linker lengthsBiochemistryJournal Article2008
Refinement of noncalorimetric determination of the change in heat capacity, Delta C-p, of protein unfolding and validation across a wide temperature rangeProteins-Structure Function and BioinformaticsJournal Article2008
Inactivation of Thrombomodulin by ionizing radiation in a cell-free system: Possible implications for radiation responses in vascular endotheliumRadiation ResearchJournal Article2008
Thermal denaturations of staphylococcal nuclease wild-type and mutants monitored by fluorescence and circular dichroism are similar: Lack of evidence for other than a two state thermal denaturationBiophysical ChemistryJournal Article2007
High apparent dielectric constant inside a protein reflects structural reorganization coupled to the ionization of an internal AspBiophysical JournalJournal Article2007
Does the oxidation of methionine in thrombomodulin contribute to the hypercoaguable state of smokers and diabetics?Medical HypothesesJournal Article2007
Development of an immunoassay for oxidized or unoxidized forms of thrombomodulinFASEB JournalJournal Article2006
Protein surface hydration mapped by site-specific mutationsProceedings of the National Academy of Sciences of the United States of AmericaJournal Article2006
Oxidation of buried cysteines is slow and an insignificant factor in the structural destabilization of staphylococcal nuclease caused by H2O2 exposureAmino AcidsJournal Article2004
Proteins with simplified hydrophobic cores compared to other packing mutantsBiophysical ChemistryJournal Article2004
Crystallographic study of water penetration and internal hydration in mutants of staphylococcal nuclease with internal ionizable residuesBiophysical JournalAbstract2004
Replacement of staphylococcal nuclease hydrophobic core residues with those from thermophilic homologues indicates packing is improved in some thermostable proteinsJournal of Molecular BiologyJournal Article2004
Large scale comparison of thermal and chemical denaturation behavior: Mutational change in protein unfolding cooperativity caused by changes in native state interactionsProtein ScienceConference Proceedings2004
Changes in stability upon charge reversal and neutralization substitution in staphylococcal nuclease are dominated by favorable electrostatic effectBiochemistryJournal Article2003
Experimental pK(a) values of buried residues: Analysis with continuum methods and role of water penetrationBiophysical JournalJournal Article2002
Investigation of biological, chemical and physical processes on and in planetary surfaces by laboratory simulationPlanetary and Space ScienceJournal Article2002
Energetics of side chain packing in staphylococcal nuclease assessed by exchange of valines, isoleucines, and leucinesBiochemistryJournal Article2001
Energetics of side chain packing in staphylococcal nuclease assessed by systematic double mutant cyclesBiochemistryJournal Article2001
Higher-order packing interactions in triple and quadruple mutants of staphylococcal nucleaseBiochemistryJournal Article2001
Packing is a key selection factor in the evolution of protein hydrophobic coresBiochemistryJournal Article2001
An initial rise in the time-resolved fluorescence anisotropy of the single tryptophan residue of the single tryptophan of the pHS/V66D mutant of Staphylococcal nuclease.Biophysical JournalAbstract2001
Structural analysis of core mutants and solvent-exposed mutants in the major alpha-helix of Staphylococcal nucleaseBiophysical JournalAbstract2001
Comparing the effect on protein stability of methionine oxidation versus mutagenesis: steps toward engineering oxidative resistance in proteinsProtein EngineeringJournal Article2001
Contribution of hydrogen bonding to the structure and stability of staphylococcal nuclease.Abstracts of Papers, 219th ACS National Meeting, San Francisco, CA, March 26-30, 2000Abstract2000
Additivity of double mutant stability effects in the core of staphylococcal nuclease.Biophysical JournalAbstract2000
Experimental measurement of the apparent dielectric effect in the interior of staphylococcal nuclease with buried charges: Implications for continuum electrostatic calculationsBiophysical JournalAbstract2000
High apparent dielectric constants in the interior of a protein reflect water penetrationBiophysical JournalJournal Article2000
Increasing the thermostability or staphylococcal nuclease: Implications for the origin of protein thermostability.Biophysical JournalAbstract2000
Increasing the thermostability of staphylococcal nuclease: Implications for the origin of protein thermostabilityJournal of Molecular BiologyJournal Article2000
The effects of multiple mutations on the protein folding and stability of staphylococcal nuclease.Abstracts of Papers, 217th ACS National Meeting, Anaheim, CA, March 21-25, 1999Abstract1999
High apparent dielectric constants inside proteins reflect water penetration.Biophysical JournalAbstract1999
Stability effects of increasing the hydrophobicity of solvent-exposed side chains in staphylococcal nucleaseBiochemistryJournal Article1998
Altering the sensitivity of protein to oxidative damage.Biophysical JournalAbstract1998
Multiple, simultaneous substitutions in the hydrophobic core of staphylococcal nuclease.Biophysical JournalAbstract1998
Solvent penetration may be responsible for the high dielectric constant inside a proteinBiophysical JournalAbstract1998
Stability effects of increasing the hydrophobicity of solvent exposed side chains in staphylococcal nuclease.Biophysical JournalAbstract1998
Substitutions of leucines with isoleucine and valine: Side chain packing in staphylococcal nuclease.Biophysical JournalAbstract1998
The pK(a) of buried ionizable groups in staph nuclease: An experimental measure of the dielectric constant of a protein interior.Biophysical JournalAbstract1998
Application of automated methods for determination of protein conformational stabilityMethods in EnzymologyBook, Section1998
Experimental measurement of the effective dielectric in the hydrophobic core of a proteinBiophysical ChemistryJournal Article1997
Protein-protein interactions: Interface structure, binding thermodynamics, and mutational analysisChemical ReviewsJournal Article1997
The M32L substitution of staphylococcal nuclease: Disagreement between theoretical prediction and experimental protein stabilityJournal of Molecular BiologyJournal Article1996
Mustard gas crosslinking of proteins through preferential alkylation of cysteinesJournal of Protein ChemistryJournal Article1996
Instrumentation for Automated-Determination of Protein StabilityAnalytical BiochemistryJournal Article1995
Energetic Contribution of Side-Chain Hydrogen-Bonding to the Stability of Staphylococcal NucleaseBiochemistryJournal Article1995
Chemically Cross-Linked Protein Dimers - Stability and Denaturation EffectsProtein ScienceJournal Article1995
Empirical-Evaluation of the Influence of Side-Chains on the Conformational Entropy of the Polypeptide BackboneProteins-Structure Function and GeneticsJournal Article1995
A Simplified Protocol for Isolation and Characterization of Ssm13 Dna Templates for use in Dideoxy SequencingBioTechniquesJournal Article1993